Group

PCR, polymerase chain reaction

Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.

Description

Premixes for PCR contain dNTPs and MgCl. A PCR premix is much easier for DNA amplification than standard TAQ polymerase.A primer is a strand of short nucleic acid sequences (generally about 10 base pairs) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand